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Primer & Probe Design Rules

 

In recent years, research has shown that modified nucleic acid chemistries such as LNA overcome the hybridization limitations of native DNA and RNA. Integration of modified chemistries into primers and probes, and the application of accurate biophysical design rules for this integration, improves the specificity and stability of oligonucleotide hybridization. This is especially true in problematic sequence contexts such as:
   
 

AT or GC rich sequences
 

Sequence locations that have hairpins and cross-hybridizations
 

Gene sequences that are repeated in the genome
   
Celadon has published original research on LNA design rules. ProbeITy software is able to apply modified nucleic acid technology to one or all of the oligonucleotides in the assay.

 

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